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  • Title: Importance of sites of tracer administration and blood sampling in relation to leucine metabolism. Practical considerations.
    Author: Pell JM, Caldarone EM, Bergman EN.
    Journal: Biochem J; 1983 Sep 15; 214(3):1015-8. PubMed ID: 6626152.
    Abstract:
    For the same infusion site of L-[1-14C]leucine, sampling downstream of arterial blood underestimates leucine turnover, whereas sampling of venous blood overestimates turnover. Further, the lungs release a small but consistent amount of leucine into the blood. Unlabelled leucine also is produced by the portal-drained viscera, and some is removed immediately by the liver. These sources of leucine should thus be considered in turnover calculations.
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