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  • Title: Preparative high-performance liquid affinity chromatography.
    Author: Small DA, Atkinson T, Lowe CR.
    Journal: J Chromatogr; 1983 Aug 26; 266():151-6. PubMed ID: 6630345.
    Abstract:
    The reactive triazine dye, Procion Blue MX-R, has been covalently attached to preparative-grade silica and used for the large-scale purification of rabbit muscle lactate dehydrogenase by high-performance liquid affinity chromatography (HPLAC). Purified dye was coupled directly to glycol-silylated silica via the reactive triazine ring to yield an adsorbent containing 12 mumol dye/g silica. Essentially homogeneous lactate dehydrogenase in 80% overall yield was obtained from crude extracts. Thus this report demonstrates the potential for adapting the speed of operation and resolution shown for triazine dye-HPLAC in analytical applications to preparative protein purification.
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