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  • Title: Analysis of porphyrin precursors, 5-aminolevulinic acid derivatives by isotachophoresis.
    Author: Fukae R, Shioi Y, Sasa T.
    Journal: Anal Biochem; 1983 Aug; 133(1):190-6. PubMed ID: 6638479.
    Abstract:
    Porphyrin precursors, 5-aminolevulinic acid (ALA), 4,5-dioxovaleric acid (DOVA), and porphobilinogen (PBG) can be simultaneously and conveniently analyzed by isotachophoresis. The quantitative analysis requires a minimum of 12 nmol for ALA, 4 nmol for DOVA, and 35 nmol for PBG. The reproducibility determined as coefficient of variation for qualitative and quantitative determination is within 4 and 5%, respectively. Isotachophoresis permits simple, efficient and quantitative detection, and identification of ALA derivatives in a mixture.
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