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  • Title: A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.
    Author: Kyte J, Rodriguez H.
    Journal: Anal Biochem; 1983 Sep; 133(2):515-22. PubMed ID: 6638511.
    Abstract:
    An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sulfate. Through this combination, high resolution and clean separation, based on polymer length, are achieved.
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