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Title: One and two dimensional isoelectric focusing of lactate dehydrogenase from the tissues of ovis aries. Author: Beatty EM, Doxey DL. Journal: Comp Biochem Physiol B; 1983; 76(2):271-5. PubMed ID: 6641160. Abstract: Lactate dehydrogenase in ovine tissues was separated by electrophoresis, one dimensional isoelectric focusing (IEF) and a two dimensional technique. Tissues showed five zones of enzyme activity consisting of multiple bands after IEF. The IEF zymograms were unique for each tissue and differed from those of other species. Two dimensional separation revealed that the five zones of activity observed on IEF corresponded to the five isoenzymes separable by conventional electrophoresis.[Abstract] [Full Text] [Related] [New Search]