These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Rapid liquid chromatographic determination of aflatoxins in heavily contaminated corn.
    Author: Hutchins JE, Hagler WM.
    Journal: J Assoc Off Anal Chem; 1983 Nov; 66(6):1458-65. PubMed ID: 6643358.
    Abstract:
    A procedure is described for rapid, quantitative determination of aflatoxins B1, B2, G1, and G2 in heavily contaminated corn (greater than 500 micrograms B1/kg) from field, greenhouse, and growth chamber experiments employing artificial inoculation of corn with Aspergillus flavus. Whole kernel corn is ground to pass a 1 mm screen and mixed before extraction of a water-wetted (25 mL) 50 g subsample with 250 mL chloroform. The filtered extract is diluted 1:1 with hexane and applied to a hexane-wetted (10 mL) disposable silica cartridge. Interferences are removed with chloroform-hexane (1 + 3), and aflatoxins are quantitatively eluted with hexane-acetone (1 + 1). Aflatoxins B1 and G1 are converted to the more intensely fluorescent hemiacetals, B2a and G2a, by treatment with trifluoroacetic acid-water. Derivatized aflatoxins are separated by reverse phase liquid chromatography (LC) and quantitated fluorometrically. Compared with AOAC method I (CB) for corn, using samples containing approximately 50 and 10 000 micrograms B1/kg, agreement between methods was good at the lower level while the rapid method yielded a considerably larger mean at the higher level. A precision study of 30 replicate samples produced a coefficient of variation of 8.46% at a mean value of 1066 micrograms B1/kg. The cartridge method was developed for LC analysis of samples that contain greater than 500 micrograms aflatoxin B1/kg corn, but it may be used to quantitate as little as 10 micrograms B1/kg with no modification.
    [Abstract] [Full Text] [Related] [New Search]