These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Positional specificity of hormone-sensitive lipase from rat adipose tissue.
    Author: Fredrikson G, Belfrage P.
    Journal: J Biol Chem; 1983 Dec 10; 258(23):14253-6. PubMed ID: 6643478.
    Abstract:
    Hormone-sensitive lipase, purified from rat adipose tissue (Fredrikson, G., Strålfors, P., Nilsson, N. O., and Belfrage, P. (1981) J. Biol. Chem. 256, 6311-6320), has been incubated with tri-, di-, and monooleoyl[3H]glycerol, and the acylglycerol reaction products were isolated by thin layer chromatography on silicic acid, impregnated with boric acid. Trioleoylglycerol was hydrolyzed with the intermediate accumulation of monooleoylglycerol, mainly the 2-isomer, and a small amount of 1,2(2,3)-, but no measurable, 1,3-dioleoylglycerol. 2-Monooleoylglycerol was also the major acylglycerol reaction product from 1,2(2,3)-dioleoylglycerol hydrolysis, which occurred at a Vmax of 60% of that with the 1,3-isomer. Part of the 1(3)-monooleoylglycerols found were formed by acyl migration, but 2-ester bond cleavage was directly demonstrated by the use of 1,3-dioleoyl-2-[14C]oleoyl[3H]glycerol as substrate, and by determination of the 14C/3H ratios of the acylglycerol reaction products. Based on the hydrolysis of specific monooleoylglycerol isomers, it was estimated that the 1(3)-ester bonds of the acylglycerols were hydrolyzed 3- to 4-fold faster than the 2-ester bonds. The main lipolytic reaction sequence catalyzed by hormone-sensitive lipase is thus triacylglycerol leads to 1,2(2,3)-diacylglycerol leads to 2-monoacylglycerol. However, the preference for the 1(3)-ester bonds is less marked than that of, e.g. pancreatic and lipoprotein lipase.
    [Abstract] [Full Text] [Related] [New Search]