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  • Title: [Thiamine- and thiamine diphosphate-binding capacity of the hyaloplasmic fraction of rat liver].
    Author: Vinogradov VV, Strumilo SA, Mandrik KA, Ostrovskiĭ IuM.
    Journal: Vopr Med Khim; 1978; 24(3):383-9. PubMed ID: 664470.
    Abstract:
    Hyaloplasmic fraction of rat liver tissue did not bind 14C-thiamin and 14C-thiamin diphosphate in vitro as shown by means of gel filtration and equilibrium dialysis. All the thiamin and 2/3 of thiamin diphosphate were in vivo in free state or in a state unbound with proteins. Hyaloplasm lost readily the endogenous thiamin diphosphate during dialysis. Endogenous thiamin diphosphate (about 1/3 of the initial content), resistent to dialysis and gel filtration, was apparently included in transketolase in hyaloplasmic fraction of rat liver tissue.
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