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  • Title: Calcium ion binding by isolated tobacco mosaic virus coat protein.
    Author: Gallagher WH, Lauffer MA.
    Journal: J Mol Biol; 1983 Nov 15; 170(4):921-9. PubMed ID: 6644815.
    Abstract:
    Calcium ion titrations were performed on solutions of tobacco mosaic virus coat protein using a calcium-specific ion-exchange electrode. Isolated coat protein was found incapable of binding calcium ions under equilibrium conditions at pH values above its iso-ionic point (pH 4.3 to 4.6). However, calcium ions were found to bind to coat protein under non-equilibrium conditions, which suggests that the isolated coat protein has the proper conformation to bind calcium ions at the iso-ionic point.
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