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  • Title: A sodium-independent mechanism for L-arginine uptake by rat renal brush border membrane vesicles.
    Author: Jean T, Ripoche P, Poujeol P.
    Journal: Membr Biochem; 1983; 5(1):1-18. PubMed ID: 6645978.
    Abstract:
    L-arginine accumulation in renal cortex slices or in brush border membrane vesicles differs from that of neutral amino acids and sugars, since it can occur despite a lack of sodium in the incubation medium. L-arginine uptake was measured under different conditions in order to determine the relative importance of the Na+-independent component of this transport. In contrast to D-glucose, which was accumulated into the vesicles only in the presence of a NaCl gradient, the accumulation of L-arginine was induced by NaCl, LiCl, KCl, or choline Cl gradients. The uptakes of L-arginine and D-glucose wer proportional to the membrane potential (inside less than 0), but the presence of Na+ was not required for the L-arginine uptake. No difference was observed in Kj values of the L-arginine uptake measured with low or high concentration of salt (Li+ or Na+). Jmax were also in the same range of magnitude. An influx of D-glucose or L-alanine (Na+-cotransported molecules) induced an increase of the sodium content in vesicles equilibrated with 22Na; the L-arginine uptake did not induce it. These different results suggest that the electrical potential and not the chemical gradient was the major driving force for the L-arginine uptake.
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