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  • Title: Co-purification of coenzyme-dependent enzymes by affinity chromatography.
    Author: Lee CY.
    Journal: Mol Cell Biochem; 1983; 57(1):27-40. PubMed ID: 6646117.
    Abstract:
    Recent developments in general ligand affinity chromatography have greatly facilitated the purification of numerous coenzyme-dependent enzymes in practical scale. By immobilizing various derivatives of coenzymes (NAD+, NADP+, ATP and coenzyme A) on Sepharose, these general ligand affinity columns have the potential of enriching as many as 30% of the two thousand known enzymes as well as their isozymic forms, if they are applied in combination with the conventional procedures.
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