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  • Title: [Sedimentation of alkaline lysates of irradiated rat bone marrow cells].
    Author: Tronov VA, Provotorov AV, Shagalov LB.
    Journal: Radiobiologiia; 1983; 23(5):599-602. PubMed ID: 6647773.
    Abstract:
    A method has been developed for registration of sedimentation diagrams of alkaline lysates of mammalian cells based on measuring UV-absorption of fractions of linear sucrose gradient during its passage down the flow of UV-cord. DNA sedimentation in alkaline lysates of irradiated bone marrow cells of rats was analyzed. The ability of these cells to repair single-strand breaks during the postirradiation incubation in buffer at 37 degrees C was demonstrated. The proposed method could be applied for screening the compounds affecting the damage and repair of DNA in a cell.
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