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  • Title: Multi-site phosphorylation of branched-chain 2-oxoacid dehydrogenase complex within mitochondria isolated from rat liver, kidney and heart.
    Author: Cook KG, Lawson R, Yeaman SJ.
    Journal: FEBS Lett; 1983 Nov 28; 164(1):85-8. PubMed ID: 6653787.
    Abstract:
    The alpha subunit of the E1 component of branched-chain 2-oxoacid dehydrogenase complex becomes rapidly phosphorylated in rat liver, kidney and heart mitochondria incubated in the presence of succinate and [32P]phosphate. Peptide mapping of tryptic digests of the phosphorylated alpha subunit indicates that 3 distinct sites are phosphorylated, as has been reported previously by us for phosphorylation in vitro of highly purified complex.
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