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  • Title: Determination of coumarin anticoagulant rodenticide residues in animal tissue by high-performance liquid chromatography. II. fluorescence detection using ion-pair chromatography.
    Author: Hunter K.
    Journal: J Chromatogr; 1983 Nov 18; 270():277-83. PubMed ID: 6655020.
    Abstract:
    A high-performance liquid chromatographic method was developed for the determination of warfarin, coumatetralyl, bromadiolone, difenacoum and brodifacoum in animal tissues using fluorescence detection. Ion-pair chromatography, with the tetrabutylammmonium ion as counter-ion, was used to take full advantage of their native fluorescence. Detection limits in liver tissue after gel permeation clean-up were 0.002 mg kg-1 for coumatetralyl, difenacoum and bromdifacoum, 0.008 mg kg-1 for bromadiolone, and 0.01 mg kg-1 for warfarin.
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