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  • Title: Purification of barley yellow dwarf virus by gel filtration on Sephacryl S-1000 Superfine.
    Author: Hewish DR, Shukla DD.
    Journal: J Virol Methods; 1983 Oct; 7(4):223-8. PubMed ID: 6655024.
    Abstract:
    Barley yellow dwarf virus was purified from infected oats using cellulase to assist virus extraction, clarification of the extract with chloroform-butanol, precipitation of virus by polyethylene glycol and gel filtration of the resulting suspension on Sephacryl S-1000 Superfine. The virus was further purified by sucrose density gradient centrifugation. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electron microscopy showed that the virus preparation is of sufficient purity for biochemical investigation. A yield of approximately 0.8 mg virus/kg of infected leaf was obtained. The technique is simple and less time-consuming than conventional centrifugation procedures and can be used routinely for purification of a wide range of spherical plant viruses.
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