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  • Title: A simple method for testosterone measurement by high-performance liquid chromatography-UV spectrophotometer.
    Author: Yamada K, Aizawa Y.
    Journal: J Pharmacol Methods; 1983 Nov; 10(3):167-73. PubMed ID: 6656261.
    Abstract:
    A simple, rapid, and reliable method for the separation and quantitation of testosterone in biological samples by a high-performance liquid chromatography-UV spectrophotometer has been developed. Separation of testosterone and prednisolone (I.S.) by HPLC was achieved in less than 3 min on a micro silica gel column using isocratic elution with hexane:chloroform:methanol (3:1:1) as the mobile phase. As little as 5 ng testosterone in biological samples can be estimated accurately by this method. This method seems to provide a new technique for the study of endocrinological pharmacology in animals and human.
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