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  • Title: Selective isolation of tryptophan-containing peptides by hydrophobicity modulation.
    Author: Sasagawa T, Titani K, Walsh KA.
    Journal: Anal Biochem; 1983 Oct 01; 134(1):224-9. PubMed ID: 6660492.
    Abstract:
    Tryptophan-containing peptides are selectively isolated from complex digests by taking advantage of changes in hydrophobicity and chromatographic mobility induced by reaction with o-nitrophenylsulfenyl chloride. The peptides are first located in crude fractions by monitoring the fluorescence during high-performance liquid chromatography and then chemically modified to facilitate their separation from contaminants during subsequent rechromatography.
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