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  • Title: The pathway of ketogenesis in rumen epithelium of the sheep.
    Author: Leighton B, Nicholas AR, Pogson CI.
    Journal: Biochem J; 1983 Dec 15; 216(3):769-72. PubMed ID: 6667266.
    Abstract:
    A method for the fractionation of sheep rumen epithelium with limited mitochondrial breakage is described. The distributions of the enzymes of the 3-hydroxy-3-methylglutaryl-CoA pathway of ketogenesis indicate that this process is exclusively mitochondrial. Enzyme activities are sufficient to account for the ketogenic rates found in vivo. The failure of (-)-hydroxycitrate to block ketogenic flux supports this view. 3-Hydroxybutyrate dehydrogenase activity is largely associated with particulate material in the mitochondrial fraction. ATP citrate lyase activity was found, with appreciable acetoacetyl-CoA thiolase and 3-hydroxy-3-methylglutaryl-CoA synthase in the cytoplasmic fraction.
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