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  • Title: Induction by 5-fluorouracil of a major phase difference in the circadian profiles of DNA synthesis between the Ehrlich ascites carcinoma and five normal organs.
    Author: Burns ER, Beland SS.
    Journal: Cancer Lett; 1983 Sep; 20(2):235-9. PubMed ID: 6667462.
    Abstract:
    DNA synthetic activity (DNA-SA) was measured by the incorporation of tritiated thymidine (TDR) into chemically isolated DNA. DNA-SA was monitored every 3 h over a 60-h period in the Ehrlich ascites carcinoma (EAC), tongue, bone marrow, ileum, stomach and rectum in mice which had received 50 mg/kg 5-fluorouracil (FU) at 17:00 h. FU was given in an attempt to induce major phase differences in the circadian profiles of DNA-SA between the EAC and the normal organs. Subsequent to the FU treatment, a time period 15 h in duration, was identified when DNA-SA in the EAC was at or near a maximal level (125-190 cpm/micrograms DNA) whereas DNA-SA in all 5 normal organs was at or near a minimal level (less than 60 cpm/micrograms DNA). Induction of such quantitative (actual levels of DNA-SA) and qualitative (180 degrees circadian phase change) differences between the normal and neoplastic cells in the same host should be useful in designing a more effective chemotherapeutic protocol for this tumor-host situation.
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