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  • Title: beta-D-Mannosidase from human placenta: properties and partial purification.
    Author: Noeske C, Mersmann G.
    Journal: Hoppe Seylers Z Physiol Chem; 1983 Dec; 364(12):1645-51. PubMed ID: 6667923.
    Abstract:
    beta-D-Mannosidase was partially purified (108-fold) from human placenta by ammonium sulfate precipitation, Concanavalin A-Sepharose affinity chromatography, gel filtration and hydroxylapatite chromatography. Further attempts to purify the enzyme by several conventional methods failed due to loss of activity. The stability of beta-mannosidase, the effect of several compounds on its activity and some physico-chemical and kinetic parameters were investigated.
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