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Pubmed for Handhelds

PUBMED FOR HANDHELDS

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  • Title: Horse lymphocyte cell synchronization: improved technique for chromosome analysis.
    Author: Märki U, Osterhoff DR.
    Journal: J S Afr Vet Assoc; 1983 Dec; 54(4):223-4. PubMed ID: 6668569.
    Abstract:
    A method using methotrexate for horse lymphocyte cell synchronization and thymidine for incorporation into DNA replication is described. This method provides a powerful technique for the study of chromosomal abnormalities and detailed analysis of chromosomal replication patterns. The determination of horse karyotypes with many similar chromosomes needs a special method which reveals the numerous and informative stages of the cell cycle. Horse lymphocyte cell cultures treated with colcemid (20 min) and harvested 6 hours after the release of the 17 hour-block with methotrexate show the best results for exact identification and analysis of horse chromosomes.
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