These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Inhibition of the in vitro formation of irreversibly sickled cells by cepharanthine.
    Author: Ohnishi ST.
    Journal: Br J Haematol; 1983 Dec; 55(4):665-71. PubMed ID: 6671086.
    Abstract:
    A method was developed to prepare irreversibly sickled cells (ISC) in vitro under a physiological condition. By exposing sickle red cells to repeated deoxygenation-reoxygenation cycles for 15 h at 37 degrees C, 20-30% of the red cells formed ISC. These cells were separated from biconcave-shaped cells by a gradient density centrifugation. The percentage of the formation of ISC was determined spectrophotometrically after cells were haemolysed. Cepharanthine, a bisbenzylisoquinoline alkaloid, was found to inhibit this in vitro formation of irreversibly sickled cells by 50% at 15 microns. This concentration was much lower than that required to inhibit the in vitro sickling.
    [Abstract] [Full Text] [Related] [New Search]