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  • Title: Latex agglutination test to identify coagulase, clumping factor and protein A produced by staphylococci.
    Author: Wang D, Bortolussi R, Noble M.
    Journal: Clin Invest Med; 1983; 6(4):301-4. PubMed ID: 6671360.
    Abstract:
    Since a rapid latex agglutination method for identifying Staphylococcus aureus has recently been described, we attempted to identify which factors in staphylococci produce a positive reaction so as to indicate the possible applications and limitations of the test. Latex particles prepared with human plasma (latex-plasma), immune serum globulin (ISG) (latex-ISG) or bovine fibrinogen (latex-fibrinogen) were evaluated. Purified protein A or staphylococcal colonies possessing protein A caused latex-ISG and latex-plasma to agglutinate. Colonies which had clumping factor (CF) caused latex-fibrinogen and latex-plasma to agglutinate. A factor, with molecular weight and physical properties identical to staphylococcal coagulase, present in the supernatant of overnight broth inoculated with S. aureus caused latex-fibrinogen and latex-plasma to agglutinate but not latex-ISG. Thus, when latex particles are prepared using plasma, the preparation appears to identify coagulase, CF and protein A.
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