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  • Title: Hydrogen metabolism and nitrogen fixation in wild type and Nif- mutants of Rhodopseudomonas acidophila.
    Author: Siefert E, Pfennig N.
    Journal: Biochimie; 1978; 60(3):261-5. PubMed ID: 667181.
    Abstract:
    N2 fixation, C2H2 reduction and H2 production in Rhodopseudomonas acidophila DSM 137 were shown to be stoichiometrically related in ratios of 1:2.8:2.8. The highest possible H2 oxidation rate has been calculated to be about 6 fold higher in Rhodopseudomonas acidophila DSM 137 than the maximum rate of H2 production. Nif- mutants were isolated and tested; all of them had lost their ability of reduce C2H2 and to produce H2. In two nif- mutants hydrogenase activity and the capacity for autotrophic growth with H2 were also strongly diminished. Nif+ revertants not only regained their ability for C2H2 reduction and H2 production but also their full capacity for autotrophic growth with H2.
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