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  • Title: Isolation of HuIFN-beta by immunosorbent and high-pressure liquid chromatography.
    Author: Smith-Johannsen H, Tan YH.
    Journal: J Interferon Res; 1983; 3(4):473-7. PubMed ID: 6672116.
    Abstract:
    A procedure for isolating HuIFN-beta without the use of SDS or other denaturants has been developed. The procedure involves the chromatography of IFN on an antibody affinity column followed by reversed-phase HPLC. The specific activity of the resulting IFN-beta is 4 X 10(8) u/mg protein, and overall recovery is about 76%. The procedure provides a method for isolating pure HuIFN-beta in its native form with an efficiency much higher than any other method reported to date.
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