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Title: Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence. Author: Klingler W, Haupt O, von Postel G, Knuppen R. Journal: Steroids; 1983 Aug; 42(2):123-36. PubMed ID: 6673182. Abstract: 6-Oxoestriol-6-(O-carboxymethyl)oxime-aminobutylethyl- isoluminol conjugate was synthesized. This luminogenic estriol derivative enabled us to develop a solid phase immunoassay method for the determination of unconjugated estriol in serum of pregnant women by the measurement of the bound estriol-isoluminol conjugate upon oxidation with a hydrogen peroxide/microperoxidase system. The sensitivity of the assay was 700 pmol/l. Results obtained by radioimmunoassay and the described method showed good agreement (r = 0.95). The chemiluminescent method is applicable in the routine measurement of unconjugated estriol.[Abstract] [Full Text] [Related] [New Search]