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  • Title: Serological studies on volunteers inoculated experimentally with a dengue virus strain in 1943.
    Author: Okuno Y, Fukunaga T, Tadano M, Fukai K, Ikeda T, Sekii K, Ariyoshi H.
    Journal: Biken J; 1983 Dec; 26(4):161-3. PubMed ID: 6678151.
    Abstract:
    In 1943, a large dengue epidemic occurred in the Osaka district and several samples of dengue virus were isolated from patients with dengue fever by workers in this Institute. These were inoculated into human volunteers to confirm that they were dengue virus. In the present study, serum samples were collected from the volunteers who had been inoculated with dengue virus and were examined serologically. In the neutralization test, all the sera showed a higher titer against dengue type 1 virus (DEN-1) than against the other three types of dengue virus, indicating that the virus strain isolated in 1943 was DEN-1.
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