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  • Title: [Visual rhodopsin. III. Complete amino acid sequence and topography in a membrane].
    Author: Ovchinnikov IuA, Abdulaev NG, Feĭgina MIu, Artamonov ID, Bogachuk AS.
    Journal: Bioorg Khim; 1983 Oct; 9(10):1331-40. PubMed ID: 6679757.
    Abstract:
    Tryptic hydrolysis of apomembranes, BNPS-skatole cleavage of carboxymethylated rhodopsin and thermolytic digestion of native membranes were carried out to obtain the peptides necessary for the polypeptide chain reconstruction. Gel-filtration on Bio-Gel P-30 in 80% formic acid, ion-exchange and reversed-phase high performance liquid chromatography were used for the peptide isolation. A comparison of rhodopsin hydrophobicity profile with the accessibility of the polypeptide chain in native photoreceptor membranes for proteases allowed to distinguish seven alpha-helical segments and propose a model for arrangement of the protein molecule in the membrane.
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