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  • Title: [Experiments with Bacillus thuringiensis protoplasts. I. Isolation of protoplasts and their reversion to bacillary form].
    Author: Tsenin AN, Nesterenko AV, Rybchin VN, Potokin IL, Pisarevskiĭ IuS.
    Journal: Genetika; 1983 Apr; 19(4):517-24. PubMed ID: 6683209.
    Abstract:
    A method for protoplastization of crystal- and spore-forming Bacillus thuringiensis bacterian and consequent cell wall regeneration on a solid hypertonic medium is presented. Up to 50% of the protoplasts prepared were viable and formed colonies under special conditions; at the same time, less than 0,01% of the cells treated with lysozyme were resistant to the osmotic shock; bacterial autolytic system takes part in protoplasts formation. Electron microscopic studies of protoplasts and cells confirm the fact of cell wall removal and support the proposed mechanism of protoplast formation.
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