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  • Title: Effect of albumin on binding and recovery of enzymes in affinity chromatography on Cibacron Blue.
    Author: Ramadoss CS, Steczko J, Uhlig JW, Axelrod B.
    Journal: Anal Biochem; 1983 Apr 15; 130(2):481-4. PubMed ID: 6683473.
    Abstract:
    Bovine serum albumin appears to improve the specificity of Cibacron Blue F3GA in affinity chromatography of enzymes which interact with nucleotides. The action of bovine serum albumin may rest in its ability to selectively mask affinity sites in the dye, which are not specific for the nucleotide-binding region of the enzyme, while not seriously impairing binding nor its elution by nucleotides. Thus, the elution of Chlorella nitrate reductase from a Blue Sepharose chromatographic column by its coenzyme, NADH, fails, unless the column is first treated with bovine serum albumin. Such treatment also improves the recovery of some other nucleotide-binding enzymes tested.
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