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  • Title: A simple and rapid enzymatic determination of L-phenylalanine with a novel L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501.
    Author: Koyama H.
    Journal: Clin Chim Acta; 1984 Jan 31; 136(2-3):131-6. PubMed ID: 6692570.
    Abstract:
    A simple and rapid method for the determination of L-phenylalanine using Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) has been developed. This method involves the oxidative deamination of L-phenylalanine using L-phenylalanine oxidase from Pseudomonas sp. P-501, and the colorimetric determination of hydrogen peroxide formed from L-phenylalanine with 4-aminoantipyrine and N,N'-dimethylaniline in the presence of peroxidase. This method requires a smaller quantity of sample and is less time-consuming than those previously reported. The method can be used for the direct assay of serum L-phenylalanine levels without pretreatment.
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