These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Whole body nitrogen kinetics in man: determination from plasma [guanidino-15N]arginine.
    Author: Yudkoff M, Nissim I, Glassman M, Segal S.
    Journal: Clin Sci (Lond); 1984 Mar; 66(3):337-42. PubMed ID: 6692663.
    Abstract:
    Whole body nitrogen turnover and protein synthesis were calculated by the method of D. Picou & T. Taylor-Roberts [Clinical Science and Molecular Medicine (1969) 36, 283-296] except that plateau plasma enrichment of [guanidino-15N]arginine was used in place of the [15N]urea enrichment after a constant infusion of [15N]-glycine. With this approach metabolic pool turnover and protein synthesis were 637.2 +/- 73.0 mg of N day-1 kg-1 and 2964.0 +/- 409.5 mg of protein day-1 kg-1 respectively. Virtually identical isotopic enrichment in [guanidino-15N]arginine and [15N]urea were observed in a healthy young adult who took repeated oral doses of [15N]glycine for a period of 60 h: 0.47 (arginine) and 0.48 (urea) atom% excess. The turnover of glycine nitrogen and of urea, determined from the constant infusion of [15N]glycine and [13C]urea, was 66.2 +/- 3.3 mg of N day-1 kg-1 and 156.2 +/- 4.3 mg of urea day-1 kg-1 respectively. The ratio of steady-state enrichment in arginine to that in glycine, reflecting the fraction of arginine derived from glycine, was 10.5%. By using the [guanidino-15N]arginine enrichment as representative of the expected enrichment in [15N]urea at plateau, it was calculated that approximately 25% of glycine N flux is directed toward the synthesis of urea, with the remainder directed to protein and quantitatively minor products like haem and creatinine.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]