These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Lateral mobility of phospholipid and cholesterol in the human erythrocyte membrane: effects of protein-lipid interactions.
    Author: Golan DE, Alecio MR, Veatch WR, Rando RR.
    Journal: Biochemistry; 1984 Jan 17; 23(2):332-9. PubMed ID: 6696882.
    Abstract:
    The phospholipid and cholesterol derivatives N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine (NBD-PE) and N1-cholesterylcarbamoyl-N8-(7-nitro-2,1,3-benzoxadiazol-4-yl )-3,6-dioxaoctane-1 , 8-diamine (NBD-Chol), respectively, were incorporated into egg phosphatidylcholine/cholesterol multilamellar liposomes, human erythrocyte ghost membranes, and multilamellar liposomes derived from extracted human erythrocyte membrane lipids. The lateral mobility of these probes in the plane of the various membranes was measured by using the fluorescence photo-bleaching recovery technique. NBD-PE and NBD-Chol manifested identical lateral mobilities in egg phosphatidylcholine/cholesterol multilamellar liposomes over the range of temperatures from 10 to 37 degrees C and the range of cholesterol mole fractions from 0.0 to 0.5, and in erythrocyte ghost membranes and erythrocyte membrane lipid-derived multilamellar liposomes over the range of temperatures from 15 to 37 degrees C. The weak temperature dependence of the lateral diffusion coefficients of the lipid probes in both artificial and erythrocyte ghost membranes is consistent with the lack of a phase transition in any of these systems over the temperature range studied. Both NBD-PE and NBD-Chol diffuse 4-fold faster in liposomes derived from extracted erythrocyte membrane lipids (D = 8.0 X 10(-9) cm2 s-1 at 37 degrees C) than in the ghost membranes themselves (D = 2.1 X 10(-9) cm2 s-1 at 37 degrees C), suggesting a significant restriction of lipid lateral mobility by membrane protein in the human erythrocyte membrane.
    [Abstract] [Full Text] [Related] [New Search]