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  • Title: Isolation and partial characterization of DNA polymerases from Crithidia fasciculata.
    Author: Holmes AM, Cheriathundam E, Kalinski A, Chang LM.
    Journal: Mol Biochem Parasitol; 1984 Feb; 10(2):195-205. PubMed ID: 6700639.
    Abstract:
    Two types of DNA polymerase activity were partially purified from Crithidia fasciculata. The alpha-type, DNA polymerase A, was of high molecular weight and sensitive to N-ethylmaleimide, whereas the beta-type, DNA polymerase B, was of low molecular weight and resistant to N-ethylmaleimide. Phosphocellulose chromatography revealed multiple peaks of DNA polymerase A activity the properties of which, such as pH optimum, salt sensitivity, utilization of synthetic template-initiator complexes and response to DNA polymerase inhibitors were similar. The response of the C. fasciculata DNA polymerase A enzymes to some of these inhibitors and utilization of poly(rA) X oligo(dT)11 showed these enzymes to be markedly different from mammalian DNA polymerase alpha.
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