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  • Title: Immunochemical characterization of a monoclonal anti-Leb blood grouping reagent.
    Author: Messeter L, Brodin T, Chester MA, Karlsson KA, Zopf D, Lundblad A.
    Journal: Vox Sang; 1984; 46(2):66-74. PubMed ID: 6702144.
    Abstract:
    A haemagglutinating monoclonal anti-LebL antibody has been obtained from a mouse-mouse hybridoma after immunization with the Leb-active oligosaccharide lacto-N-difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme-treated erythrocytes of all ABO groups and three more were considered to be anti-LebH haemagglutinins. The remaining nine antibodies did not agglutinate red cells. The specificity of the stronger of the anti-LebL haemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides.
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