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  • Title: The kinetic mechanism of pyruvate reduction by lactate dehydrogenase from Phycomyces blakesleeanus.
    Author: Busto F, de Arriaga D, Soler J.
    Journal: Int J Biochem; 1984; 16(2):171-6. PubMed ID: 6705969.
    Abstract:
    The kinetics of pyruvate reduction by lactate dehydrogenase from Phycomyces blakesleeanus NRRL 1555 (-) have been determined at pH 6.0. Initial rate studies performed in the pyruvate reduction direction suggest that a sequential mechanism is operating. Product inhibition studies with NAD+ and L(+)-lactate are consistent with an ordered sequential mechanism if we considered that NAD+ mimics the NADH that binds cooperatively on the enzyme and also the existence of dead-end complex responsible for substrate inhibition by pyruvate at this pH value.
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