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  • Title: A quantitative fluorometric assay for detection and characterization of Fc receptors.
    Author: Schreiber AB, Hoebeke J, Bergman Y, Haimovich J, Strosberg AD.
    Journal: J Immunol; 1978 Jul; 121(1):19-23. PubMed ID: 670698.
    Abstract:
    A new quantitative fluorometric binding assay that uses fluoresceinated aggregated IgG is proposed for the study of Fc receptors. The method was compared with a radiolabeling binding assay on three well characterized murine cell lines (38C-13, EL4, and BW). The apparent association constant of the binding and the amount of aggregated IgG bound per cell at saturation were calculated. The fluorometric assay enables the detection of 5 X 10(-10) M bound aggregated IgG. Inhibition studies with monomeric IgG, reduced and alkylated aggregated IgG, and aggregated F(ab')2 fragments of IgG confirmed the specificity of the assay. Staphylococcal protein A inhibited the binding of the aggregated IgG to Fc receptors.
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