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  • Title: Determination of biogenic amines in meat by combined ion-exchange and capillary gas chromatography.
    Author: Slemr J, Beyermann K.
    Journal: J Chromatogr; 1984 Jan 20; 283():241-50. PubMed ID: 6707120.
    Abstract:
    A procedure is described for the determination of putrescine, cadaverine and histamine in meat. Crude perchloric acid extracts were pre-separated on a weakly acidic cation exchanger and the amines quantified by capillary gas chromatography. The diamines were analysed as trifluoroacetyl derivatives and histamine was converted into N alpha-trifluoroacetyl-N tau-ethoxycarbonylhistamine. The accuracy of the determination of diamines was examined by a precipitation pre-separation method and by mass fragmentometric quantification. The proposed procedure allows the sensitive, sufficiently precise and highly specific determination of putrescine and cadaverine in meat.
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