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  • Title: [Effect of the pH of the lysing solution on the sedimentation properties of the nucleoid of intact and irradiated Ehrlich ascites tumor cells].
    Author: Ingel' FI, Kutman AI, Riabchenko NI.
    Journal: Radiobiologiia; 1984; 24(1):73-6. PubMed ID: 6709847.
    Abstract:
    A study was made of the sedimentation properties of Ehrlich ascites tumor cell nucleotide obtained by cell lysis in 1.95 M NaCl, 0.1 M EDTA, 0.02 M tris, and 0.5% triton X-100, at pH 5.0 and 8.0. It was shown that the nucleotide obtained at pH 5.0 has a more compact structure than that obtained at pH 8.0. Irradiation of cells leads to relaxation of the nucleotide decreasing the rate of its sedimentation in a neutral sucrose gradient. The influence of irradiation on the sedimentation properties is more pronounced with pH 5.0 than 8.0.
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