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  • Title: Mode of binding of pyridoxal 5'-phosphate in rat liver ornithine aminotransferase.
    Author: Sanada Y, Shiotani T, Katunuma N.
    Journal: J Nutr Sci Vitaminol (Tokyo); 1978; 24(2):77-82. PubMed ID: 671110.
    Abstract:
    1. Pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate were effective for the association of apo-form II of ornithine aminotransferase [EC 2.6.1.13]; whereas other B6 derivatives, such as pyridoxal, pyridoxamine, pyridoxine and pyridoxine 5'-phosphate, had no effect on form II of this apoenzyme. 2. The pyridoxal 5'-phosphate contents of the native enzyme, and reconstituted forms I and II were determined by two different methods to be 1.5 moles, 2.5 moles and 3.3 moles of pyridoxal 5'-phosphate/mole of enzyme, respectively.
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