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  • Title: [Organotypic culture of normal and neoplastic laryngeal mucosa].
    Author: Bodo M, Pitzurra M, Sensini A, Maurizi M, Paludetti G, Fronticelli F.
    Journal: Boll Soc Ital Biol Sper; 1984 Mar 30; 60(3):611-6. PubMed ID: 6712829.
    Abstract:
    Mucous membrane's phragments from human normal and neoplastic larynx have been maintained in a organ culture system. A set of cultures has been prepared for optical microscope studies and a parallel set for incorporation studies using 3H Thymidine and 3H Leucine. The results show that there is a good incorporation of 3H Thymidine and a greater incorporation of 3H Leucine at the 7th day due to a differentiation in vitro with a new synthesis of cheratin. These data agree with the morphologic evidence of considerable horny structures in the pathological mucous membrane after 11 days at culture.
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