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  • Title: A size analysis of the adenovirus replicon.
    Author: Lally C, Dörper T, Gröger W, Antoine G, Winnacker EL.
    Journal: EMBO J; 1984 Feb; 3(2):333-7. PubMed ID: 6714222.
    Abstract:
    The linear double-stranded genome of adenovirus DNA replicates semiconservatively from two origins of replication at either of the two molecular ends. Using an in vitro replication system which is able to initiate de novo DNA synthesis we have mapped the origin of DNA replication within the terminal 19 bp of the viral genome. Our conclusions are based on the use of different natural DNA templates, i.e., adenovirus type 2 and mouse adenovirus Fl DNA. In addition, we have employed linearized plasmid DNA templates which contain cloned terminal restriction enzyme fragments as well as chemically synthesized adenovirus termini of different length.
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