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Title: Accelerated net efflux of 3-O-[14C]methylglucose in isolated fat cells. Author: Vinten J. Journal: Biochim Biophys Acta; 1984 May 30; 772(3):244-50. PubMed ID: 6722147. Abstract: A flow-tube apparatus suited for measurement of rapid efflux of sugars from adipocytes is described. Due to heterogeneity of fat cell populations, a conventional analysis of the time-course of net efflux of 3-O-methylglucose based on the integrated rate equation can produce gross errors in estimates of kinetic parameters. The half-saturation constant and maximum transport capacity for 3-O-methylglucose transport were found to be about 3-fold higher for net efflux than for equilibrium exchange flux, both in insulin-stimulated and non-stimulated adipocytes. This suggests asymmetric kinetic parameters for 3-O-methylglucose transport.[Abstract] [Full Text] [Related] [New Search]