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  • Title: Composition and protein efficiency ratio of meat samples partially defatted with petroleum ether, acetone, or ethyl ether.
    Author: Happich ML, Ackerman S, Miller AJ, Swift CE, Gumbmann MR.
    Journal: J Assoc Off Anal Chem; 1984; 67(2):265-70. PubMed ID: 6725195.
    Abstract:
    Freeze-dried beef samples were partially defatted with either petroleum ether, acetone, or ethyl ether before determination of protein efficiency ratio (PER) to study the extraction effects on the composition and protein nutritional quality of the extracted beef. Defatting a protein source, such as meat or a meat product, may often be necessary to produce a test diet that contains 10% protein and 8% fat. Amino acid, carnosine, anserine, creatine, creatinine, inosine, and proximate compositions were determined on the extracted samples. Resulting data were compared to the composition and PER data of the beef that had no solvent treatment. Although the chemical analysis data from the study showed some variation between the proteins and other nitrogenous components of the unextracted and the extracted beef, these variations were too small to affect the protein nutritional quality of the beef as measured by PER.
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