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  • Title: Quantitative analysis of hyaluronic acid by high-performance liquid chromatography of streptomyces hyaluronidase digests.
    Author: Takazono I, Tanaka Y.
    Journal: J Chromatogr; 1984 Apr 20; 288(1):167-76. PubMed ID: 6725472.
    Abstract:
    The separation and quantitative analysis of streptomyces hyaluronidase ( HAase ) degradation products of hyaluronic acid (HA) by high-performance liquid chromatography are described. The substituted 4,5-unsaturated tetrasaccharide and hexasaccharide which result from the digestion of HA are quickly separated on a silica gel (Zorbax SIL) column. The assay of HA is linear between 5 and 250 micrograms of HA. This procedure is suitable for some weakly acidic glycosaminoglycan contaminants having similar properties to those of HA.
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