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  • Title: Revised method for the quantitative determination of 5-hydroxytryptamine in human plasma by gas chromatography--mass spectrometry--selected ion monitoring.
    Author: Baba S, Utoh M, Horie M, Mori Y.
    Journal: J Chromatogr; 1984 Apr 13; 307(1):1-9. PubMed ID: 6725475.
    Abstract:
    To estimate the 5-hydroxytryptamine level in human plasma by gas chromatography--mass spectrometry--selected ion monitoring (GC--MS--SIM), a recovery method from plasma and derivatization conditions with pentafluoropropionic anhydride were investigated. By heating 5-hydroxytryptamine at 140 degrees C for 4 h in the presence of pentafluoropropionic anhydride the peak intensity of derivatized 5-hydroxytryptamine increased more than three times in comparison with the reported method. There was a marked difference in the plasma levels of 5-hydroxytryptamine obtained using the GC--MS--SIM method compared to those obtained using fluorometric assay.
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