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  • Title: [DNA-RNA cytofluorometry for a primary cancer of the stomach and choledochus].
    Author: Kamachi M, Morotomi N, Kusuzaki K, Kagawa K, Deguchi T, Okabe H, Fujimiya T, Tsuchihashi Y, Hidaka T, Takeoka O.
    Journal: Gan No Rinsho; 1984 May; 30(5):466-72. PubMed ID: 6727046.
    Abstract:
    We standardized DNA-RNA cytofluorometry with AO stain using a NIKON SPM-RFI-D instrument for cell smears. We applied this technique in a 72-year-old woman with primary double cancer of the stomach and choledochus. We first suspected malignancy of the choledochus and examined the desquamated cells in the bile cytofluorometrically to obtain by X-Y plotting the irregularly increased polyploid cells with a high percentage of cells in S phase. These polyploid cells and S phase cells were found to have an increased RNA content. Before operation, the presence of another malignancy of the stomach was also ascertained. The surgical specimens were diagnosed histopathologically as well differentiated, tubular adenocarcinoma of the stomach and moderately differentiated, tubular adenocarcinoma of the choledochus. By cytofluorometric analysis similar results were obtained, i.e., polyploidization with increase of S phase cells. Based on these results, we concluded that our method is useful for the diagnosis of alimentary tract malignancy, and that the criteria of malignancy appear to be unusual polyploidization with increased fractions of S phase cells.
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