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  • Title: [Use of Nobuto's method for controlling the immune titer of poultry against Newcastle disease].
    Author: Dimitrova E.
    Journal: Vet Med Nauki; 1984; 21(1):17-21. PubMed ID: 6730316.
    Abstract:
    The use of Nobuto 's method for blood sampling with birds and mammals, using filter paper, suggests various advantages in the practice of serologic investigations. Studies were carried out to determine the immune titer of birds against Newcastle disease. Parallel blood samples were taken routinely and after the method of Nobuto with filter paper. Serologic examination was performed through the hemagglutination-inhibition test, beta procedure, with 10 HAU /0.5 cm3. As many as five parallel experiments with blood samples from birds were conducted within the period of from the 15th to the 40th day following vaccination. The results of HI-tests on filter paper, variants a and b, proved mathematically reliable, coinciding with the HI results with the samples studied with sera (P = 0.05). Further studies of blood samples from birds after the HI, beta micromethod, are currently carried out.
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