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  • Title: A new radioimmunoassay for the midregion of canine parathyroid hormone.
    Author: Mallette LE, Tuma SN.
    Journal: Miner Electrolyte Metab; 1984; 10(1):43-7. PubMed ID: 6738461.
    Abstract:
    A new radioimmunoassay for canine PTH employs a goat antiserum against native bovine and human PTHs, with either [125I]bPTH-(41-84) or [125I] [tyr43]-hPTH-(43-68) as Tracer. The assay is monospecific for the midregion (44-68) of PTH. Assay of 40-microliters aliquots allowed detection of PTH in 20 of 22 sera from normal dogs, with these normal iPTH levels inhibiting tracer binding by 19-68%. iPTH levels rose markedly after a decrease in serum calcium ion activity induced by intravenous injection of disodium EDTA; the rise could be detected at 3 min and showed two peaks, one at 3-6 min and one at 10-15 min. After parathyroidectomy, PTH immunoreactivity disappeared rapidly from serum. After nephrectomy, iPTH increased many-fold within 12 h. The assay has a similar specificity and sensitivity to our recently described midregion assay for human PTH and should prove useful in studies of normal parathyroid physiology.
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