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  • Title: Species differences in the occurrence of copper-metallothionein in the particulate fractions of the liver of copper-loaded animals.
    Author: Mehra RK, Bremner I.
    Journal: Biochem J; 1984 Apr 15; 219(2):539-46. PubMed ID: 6743231.
    Abstract:
    Large amounts of Cu-metallothionein were obtained by 2-mercaptoethanol and sodium dodecyl sulphate extractions of the particulate fractions of the liver of pigs given high-Cu2+ diets or rats injected with Cu2+. Three isoproteins were purified from pig liver and characterized on the basis of their physicochemical properties, metal content and amino acid composition. No such pool of Cu-metallothionein was present in the liver of Cu2+-loaded sheep or of rats given Cu2+-supplemented diets.
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